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Image Search Results
Journal: Frontiers in Cell and Developmental Biology
Article Title: The Laminin Receptors Basal Cell Adhesion Molecule/Lutheran and Integrin α7β1 on Human Hematopoietic Stem Cells
doi: 10.3389/fcell.2021.675240
Figure Lengend Snippet: Immunostaining of lineage negative HSPC with the anti-α7 integrin antibody. (A) The lineage-negative CBMNC fraction fixed by cytospin centrifugation was labeled with a mouse monoclonal anti-human integrin α7 chain antibody (red) and a rabbit monoclonal anti-human CD34 antibody (green). The cells were counterstained with DAPI (blue). The merged picture shows that all CD34 + HSPC express integrin α7. Representative of three independent experiments. (B) Lin- CD34+ cells were labeled with the unconjugated integrin antibody followed by an incubation with FITC-conjugated secondary antibody, and measured by flow cytometry. Representative plot of three independent experiments. (C) Two different preparations of lin- CD34+ cell extracts were analyzed by immunoblotting. An extract from HUVEC cells was used as a positive control. In all extracts a band of 117 kb can be detected.
Article Snippet: To obtain lineage-negative (lin-) stem and progenitor cells, the CBMNC fraction was magnetically labeled with the
Techniques: Immunostaining, Centrifugation, Labeling, Incubation, Flow Cytometry, Western Blot, Positive Control